“Hemp seeds are valued for their unique nutritional and health benefits.

This study examined the impact of supercritical (sc)CO₂ extraction conditions on hemp seed oil yield, composition, antioxidant activity, and enzyme inhibition using a multivariate approach. While pressure (300-500 bar) had minimal effects, temperature (40-60 °C) and ethanol addition (0.6-1.5 %) significantly influenced oil yield.

The levels of fatty acids, tocopherols, carotenoids, chlorophylls, phenolics, and flavonoids varied independently of extraction pressure and temperature, but their extractability generally increased with ethanol concentration. The co-solvent addition also enhanced radical scavenging activity but diminished the metal-reducing and chelating properties.

Hemp seed oils inhibited enzymes linked to chronic diseases like diabetes, skin disorders, and Alzheimer’s.

Multivariate analysis grouped samples by fatty acid profile, pigment content, and bioactivity. This work provides novel insights into how scCO₂ conditions affect the chemical and biological properties of hemp seed oils.”

https://pubmed.ncbi.nlm.nih.gov/41341704

“Cannabis sativa L. (hemp) seeds have been consumed as animal feed or for human nutrition for thousands of years. The whole hemp seeds are comprised of fiber, proteins, and contain more than 30 % oil.

Hemp seed oil is abundant in polyunsaturated fatty acids (PUFAs) and exhibits a favorable omega-6 to omega-3 fatty acid (ω6/ω3) ratio of 3:1. This ratio is regarded as optimal in human nutrition, as it can significantly reduce the incidence of cardiovascular pathologies.”

“This study highlighted that scCO₂ extraction is a promising method for obtaining high-quality oil from de-hulled hemp seeds, with tunable parameters influencing both yield and bioactivity.”

https://www.sciencedirect.com/science/article/pii/S2590157525011435?via%3Dihub