Cannabidiol-derived compounds are being explored for skin applications that go beyond inflammation and pain. In this study, a CBD-thiosemicarbazone derivative showed dual activity in human skin-derived cells by inhibiting tyrosinase, a key enzyme involved in melanin production, while also providing antioxidant protection. This combination suggests potential value in conditions associated with hyperpigmentation and oxidative skin damage. The findings highlight how modified cannabinoid compounds may expand the dermatological uses of cannabis-derived molecules.
“The search for modulators of melanogenesis with improved biological compatibility remains an active area of investigation, as existing tyrosinase (TYR) inhibitors are often limited by low potency, instability, or cytotoxicity.
Here, we investigated CBD-TSC1, a cannabidiol-based thiosemicarbazone derivative, as a TYR-targeting scaffold.
Structural characterization confirmed a single, stable E-isomer, and pKa profiling together with kinetic analyses indicated reversible mixed-type inhibition of human TYR, involving interactions with both free enzyme and enzyme-substrate complexes. CBD-TSC1 exhibited higher inhibitory activity than CBD and kojic acid under the tested conditions while maintaining low cytotoxicity in G361 melanoma and HaCaT keratinocyte cell lines. In addition, CBD-TSC1 reduced intracellular oxidative stress at low micromolar concentrations. In zebrafish larvae, treatment with CBD-TSC1 resulted in a dose-dependent reduction in melanin content, comparable to that of kojic acid under identical experimental conditions, supporting an association between thiosemicarbazone modification and the observed biological activity.
Overall, CBD-TSC1 demonstrated consistent activity across biochemical, cellular, and zebrafish-based assays under the tested conditions. Although the mechanistic relationship between TYR inhibition, redox modulation, and melanogenesis regulation remains to be fully clarified, the present findings support further investigation of cannabidiol-based thiosemicarbazone derivatives as modulators of TYR-related pathways.”
https://pubmed.ncbi.nlm.nih.gov/42235859
“CBD-TSC1 is a novel cannabidiol-thiosemicarbazone hybrid with dual anti-melanogenic activity.”
“Thiosemicarbazone modification is essential for superior enzymatic and redox performance.”
https://www.sciencedirect.com/science/article/abs/pii/S0960894X26001745?via%3Dihub