Category Archives: Uncategorized

Probing the antioxidant activity of Δ9-tetrahydrocannabinol and cannabidiol in Cannabis sativa extracts.

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“Herein, we report the antioxidant activity of cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC) in pure and mixed solutions at different ratios, as well as of six different Cannabis sativa extracts containing various proportions of CBD and THC by using spectrophotometric (reducing power assay, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), hypochlorous acid (HOCl) scavenging assays) and electrochemical methods (cyclic voltammetry and differential pulse voltammetry).

The isolated cannabinoids, the different stoichiometric ratios of CBD and THC, and the natural extracts proved to have remarkable antioxidant properties in all the methods employed in this work.

The antioxidant activity of CBD and THC was compared against that of the well-defined antioxidants such as ascorbic acid (AA), resveratrol (Resv) and (-)-epigallocatechin-3-gallate (EGCG). Clear evidence of the synergistic and antagonistic effects between CBD and THC regarding to their antioxidant activities was observed.

Moreover, a good correlation was obtained between the optical and electrochemical methods, which proved that the reported experimental procedures can easily be adapted to determine the antioxidant activity of extracts from various Cannabis sativa species and related compounds.”

https://www.ncbi.nlm.nih.gov/pubmed/31318364

https://pubs.rsc.org/en/content/articlelanding/2019/AN/C9AN00890J#!divAbstract

Graphical abstract: Probing the antioxidant activity of Δ9-tetrahydrocannabinol and cannabidiol in Cannabis sativa extracts

Employment and Marijuana Use Among Washington State Adolescents Before and After Legalization of Retail Marijuana

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 Journal of Adolescent Health Home“The purpose of the study was to describe associations between employment and marijuana use among adolescents 2 years before passage of 2012 ballot initiative and 2 years after the implementation of retail recreational marijuana sales took place in Washington.

Working adolescents in all grades had higher prevalence of recent marijuana use compared with nonworking adolescents.

Working youth were more likely to use marijuana before and after Washington’s legalization of retail marijuana.”

https://www.jahonline.org/article/S1054-139X(19)30020-5/fulltext

“Study shows working teens more likely to try marijuana. Employed adolescents are more likely to use marijuana than those who don’t work, according to a study recently published in the Journal of Adolescent Health.”
“Teens with jobs are more likely to use cannabis than those who aren’t employed: study” https://www.thegrowthop.com/cannabis-news/teens-with-jobs-are-more-likely-to-use-cannabis-than-those-who-arent-employed-study

Association of Marijuana Laws With Teen Marijuana Use

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“In the United States, 33 states and the District of Columbia have passed medical marijuana laws (MMLs), while 10 states and the District of Columbia have legalized the recreational use of marijuana.

A 2018 meta-analysis concluded that the results from previous studies do not lend support to the hypothesis that MMLs increase marijuana use among youth, while the evidence on the effects of recreational marijuana laws (RMLs) is mixed.

Here, we report estimates of the association between the legalization of marijuana and its use, simultaneously considering both MMLs and RMLs.

Consistent with the results of previous researchers, there was no evidence that the legalization of medical marijuana encourages marijuana use among youth.

Moreover, the estimates reported in the Table showed that marijuana use among youth may actually decline after legalization for recreational purposes.

This latter result is consistent with findings by Dilley et al and with the argument that it is more difficult for teenagers to obtain marijuana as drug dealers are replaced by licensed dispensaries that require proof of age.”

https://jamanetwork.com/journals/jamapediatrics/fullarticle/2737637?guestAccessKey=5e4e41eb-ec96-4641-86f9-b5c89cc7cc48&utm_source=For_The_Media&utm_medium=referral&utm_campaign=ftm_links&utm_content=tfl&utm_term=070819

“New JAMA study shows legalizing pot might discourage teen use”  https://www.cnbc.com/2019/07/08/new-jama-study-shows-legalizing-pot-might-discourage-teen-use.html

“Recreational marijuana legalization tied to decline in teens using pot, study says”  https://www.cnn.com/2019/07/08/health/recreational-marijuana-laws-teens-study/index.html

“Recreational marijuana legalization tied to decline in teens using pot, study says”  https://wtvr.com/2019/07/08/recreational-marijuana-legalization-tied-to-decline-in-teens-using-pot-study-says/

Model-based analysis on systemic availability of coadministered cannabinoids after controlled vaporised administration.

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“The most important two medicinal cannabinoids are Δ9 -tetrahydrocannabinol (THC) and cannabidiol (CBD).

The results observed in this study are useful for guiding future pharmacokinetic studies of medicinal cannabinoids, and for development of dosing guidelines for medical use of cannabis in the ‘real world’ setting.”

https://www.ncbi.nlm.nih.gov/pubmed/31264294

https://onlinelibrary.wiley.com/doi/abs/10.1111/imj.14415

[Topical cannabinoid agonists. An effective new possibility for treating chronic pruritus].

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“Chronic, therapy-resistant pruritus often fails to respond to standard measures so new therapeutic approaches are needed.

Recently, the expression of cannabinoid receptors on cutaneous sensory nerve fibers was described, so cannabinoid agonists seem a rational therapeutic option for pruritus.

RESULTS:

In 14/22 patients a good antipruritic effect could be documented. The average reduction in itch was 86.4%. The therapy was well-tolerated by all patients; neither burning burn nor contact dermatitis was observed.

CONCLUSIONS:

Topical cannabinoid agonists represent an new effective and well-tolerated therapy for refractory itching of various origins. Creams with a higher concentration may be even more effective with broader indications.”

https://www.ncbi.nlm.nih.gov/pubmed/16874533

https://link.springer.com/article/10.1007%2Fs00105-006-1180-1

“Cannabinoids for the treatment of chronic refractory pruritus.”  https://www.ncbi.nlm.nih.gov/pubmed/31264498

Cannabis sativa L. extract and cannabidiol inhibit in vitro mediators of skin inflammation and wound injury.

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Publication cover image“The present study investigates the potential effect of a Cannabis sativa L. ethanolic extract standardized in cannabidiol as antiinflammatory agent in the skin. The extract inhibited the release of mediators of inflammation involved in wound healing and inflammatory processes occurring in the skin. Cannabis extract and cannabidiol showed different effects on the release of interleukin-8 and vascular endothelial growth factor, which are both mediators whose genes are dependent on NF-κB. Our findings provide new insights into the potential effect of Cannabis extracts against inflammation-based skin diseases.” https://www.ncbi.nlm.nih.gov/pubmed/31250491

https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.6400

“The endocannabinoid system of the skin in health and disease: novel perspectives and therapeutic opportunities” https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2757311/

“The endocannabinoid system of the skin. A potential approach for the treatment of skin disorders” https://www.sciencedirect.com/science/article/abs/pii/S0006295218303484

“Cannabinoid system in the skin – a possible target for future therapies in dermatology.”   https://www.ncbi.nlm.nih.gov/pubmed/19664006

“Extracts of the hemp plant cannabis are traditionally used as a popular remedy against inflammation.” https://medicalxpress.com/news/2007-06-cannabinoids-human-body-anti-inflammatory-effect.html

[Survey of neurologists regarding their attitudes toward medicinal cannabis and the effects of evidence-based cannabis education].

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“While more than half of the respondents in both groups showed some acceptance toward the usage of cannabis for research purposes, there was a stronger tendency to accept the use of cannabis for medical purposes in the informed group. Since this acceptance was more often displayed by respondents who had adequate knowledge of the medical use of cannabis, this suggests that providing information on cannabis is useful in promoting acceptance. The result of the survey indicated that a portion of neurologists acknowledges the usefulness of cannabis, and that one’s receptivity toward cannabis can be improved if adequate information is provided about cannabis.”   https://www.ncbi.nlm.nih.gov/pubmed/31243253

https://www.jstage.jst.go.jp/article/clinicalneurol/advpub/0/advpub_cn-001299/_article/-char/ja/

Cannabinoid receptor 1 (CB1R) expression in rat dental pulp

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Oral Science International“Accumulating evidence supports the role of the cannabinoid system in providing an antinociceptive effect in various painful conditions.

This effect is mediated through the Cannabinoid receptor 1 (CB1R) expressed on nociceptive afferent nerve terminals.

To investigate whether this receptor plays a similar role in dental pain, we studied the presence and distribution of CB1R in rat dental pulp.

CB1R was present on nerve fibers in rat dental pulp and possibly plays a role in dental pain mechanisms.

Interestingly, CB1R has recently been demonstrated in human dental pulp.

This strongly suggests that CB1R could be a therapeutic target for dental pain management.”

https://www.sciencedirect.com/science/article/pii/S1348864312000031

Cannabis and cannabinoids on treatment of inflammation: a patent review

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The inflammatory process is a physiological response to a vast number harmful stimulus that takes place in order to restore homeostasis. Many drugs used in pharmacotherapy are effective to control inflammatory responses, however there is a range of adverse effects attributed to steroidal and non-steroidal anti-inflammatory drugs (NSAIDs).

In this sense, herbal medicine and derivatives gain more adepts because of their effectiveness and safety, showing the importance of medicinal plants, especially the Cannabis genus and the cannabinoid derivatives.
The aim of this prospection was to identify data related to patents involving Cannabis and cannabinoids for the treatment of inflammation.
A total of 370 patents were found, of which 17 patents met the inclusion criteria.
Although reports show synergistic effects of the plant components, patents involving Cannabis and cannabinoids focus on isolated substances (CBD e THC). However, patents related to Cannabis and cannabinoids are promising for future use of the plant or its derivatives on the treatment of inflammation.”
“Cannabis-based drugs have been shown to be effective in inflammatory diseases.” https://www.ncbi.nlm.nih.gov/pubmed/29110674
“Cannabinoid-based drugs as anti-inflammatory therapeutics.” http://www.ncbi.nlm.nih.gov/pubmed/15864274
http://www.thctotalhealthcare.com/tag/anti-inflammatory/